@article {245, title = {Angiographic and histological characterization of PFA-induced coronary spasm: Differential effect of two waveforms}, journal = {Heart Rhythm}, volume = {(In Press, Journal Pre-proof)}, year = {2026}, doi = {10.1016/j.hrthm.2026.03.1904}, url = {https://doi.org/10.1016/j.hrthm.2026.03.1904}, author = {Gerard Amor{\'o}s-Figueras and Q. Castellv{\'\i} and Sergi Casabella-Ramon and Marc Soriano-Amores and {\'A}lvaro Borachok and del Pino, Pablo and Zoraida Moreno-Weidmann and Antoni Ivorra and Jose M. Guerra} } @article {247, title = {Frequency-induced fatigue in electrically stimulated sheep hindlimb muscles}, journal = {Biomedical Physics \& Engineering Express}, volume = {(accepted manuscript online)}, year = {2026}, doi = {10.1088/2057-1976/ae6346}, url = {https://iopscience.iop.org/article/10.1088/2057-1976/ae6346}, author = {Mateu-Yus, Berta and Ana Garcia-Maestre and Aracelys Garc{\'\i}a-Moreno and Antoni Ivorra} } @article {246, title = {Next Generation of Brain-Computer Interfaces: A Proposal for Spikes Detection Based on CDMA and Power Domain Nonorthogonal Multiple Access Techniques}, journal = {IEEE Transactions on Human-Machine Systems}, volume = {(Early Access)}, year = {2026}, doi = {10.1109/THMS.2026.3663792}, url = {https://ieeexplore.ieee.org/abstract/document/11415676}, author = {Jesus Minguillon and Lopez-Gordo, Miguel Angel and Pablo Martinez-Ca{\~n}ada} } @article {238, title = {Atrial fibrillation and flutter conversion with pulsed electric field delivery: preclinical proof of concept}, journal = {Journal of Interventional Cardiac Electrophysiology}, year = {2025}, doi = {10.1007/s10840-025-02115-7}, url = {https://doi.org/10.1007/s10840-025-02115-7}, author = {Tan, Nicholas Y. and Del-Carpio-Munoz, Freddy and Tri, Jason A. and Taro Koya and Naoto Otsuka and DeSimone, Christopher V. and Suraj Yalamuri and Elad Maor and Q. Castellv{\'\i} and Antoni Ivorra and Damijan Miklav{\v c}i{\v c} and Samuel Asirvatham} } @article {240, title = {The Biomedical Engineer{\textquoteright}s Pledge: Overview and Context}, journal = {Medical \& Biological Engineering \& Computing}, volume = {(Published online)}, year = {2025}, doi = {10.1007/s11517-025-03443-6}, url = {https://doi.org/10.1007/s11517-025-03443-6}, author = {Antoni Ivorra and Txetxu Aus{\'\i}n and Laura Becerra-Fajardo and del Ama, Antonio J. and Jesus Minguillon and Aracelys Garc{\'\i}a-Moreno and Jordi Aguil{\'o} and Filipe O. Barroso and Bijnens, Bart and Oscar Camara and Sara Capdevila and Castellanos-Fernandez, Roger and Rafael Davalos and Jean-Louis Divoux and Ahmed Eladly and Dario Farina and Garc{\'\i}a-Hombravella, Carla and Gonz{\'a}lez-L{\'o}pez, Raquel and C{\'e}sar Gonz{\'a}lez and Jordi Gr{\'\i}fols and Felipe Maglietti and Shahid Malik and Elad Maor and Guillermo Marshall and Mateu-Yus, Berta and Lluis M. Mir and Moreno, Juan C. and Xavier Navarro and N{\'u}ria Noguera and Andr{\'e}s Ozaita and Gemma Piella and Pons, Jos{\'e} Luis and Rita Quesada and Pilar Rivera-Gil and Boris Rubinsky and Ruiz-Garcia, Aurelio and Ruiz-Vargas, Albert and S{\'a}nchez-S{\'a}nchez, Maria and Andreas Schneider and Ting Shu and Villa-Sanz, Rosa and Bing Zhang and Gema Revuelta} } @article {243, title = {Local modifications in tissular conditions before PFA increases acute lesion size}, journal = {Heart Rhythm O2}, volume = {(In press)}, year = {2025}, doi = {10.1016/j.hroo.2025.11.021 }, url = {https://www.heartrhythmopen.com/article/S2666-5018(25)00567-7/fulltext}, author = {Gerard Amor{\'o}s-Figueras and Sergi Casabella-Ramon and Zoraida Moreno-Weidmann and Q. Castellv{\'\i} and Antoni Ivorra and Tomas Garcia-Sanchez and Jose M. Guerra} } @article {233, title = {Is a single lethal electric field threshold sufficient to characterize the lesion size in computational modeling of cardiac pulsed field ablation?}, journal = {Heart Rhythm O2}, volume = {6}, year = {2025}, pages = {671-677}, chapter = {671}, doi = {10.1016/j.hroo.2025.02.014}, url = {https://doi.org/10.1016/j.hroo.2025.02.014}, author = {Argyrios Petras and Gerard Amor{\'o}s-Figueras and Zoraida Moreno-Weidmann and Tomas Garcia-Sanchez and David Vilad{\'e}s Medel and Antoni Ivorra and Jose M. Guerra and Luca Gerardo-Giorda} } @article {231, title = {Changes in local endocardial electrograms immediately after PFA show dose-dependent variations}, journal = {EP Europace}, volume = {26}, year = {2024}, pages = {euae102.755}, chapter = {euae102.755}, doi = {10.1093/europace/euae102.755}, url = {https://academic.oup.com/europace/article/26/Supplement_1/euae102.755/7681509}, author = {Gerard Amor{\'o}s-Figueras and Zoraida Moreno-Weidmann and Sergi Casabella-Ramon and Antoni Ivorra and Jose M. Guerra and Tomas Garcia-Sanchez} } @article {227, title = {First-in-human demonstration of floating EMG sensors and stimulators wirelessly powered and operated by volume conduction}, journal = {Journal of NeuroEngineering and Rehabilitation}, volume = {21}, year = {2024}, pages = {4}, chapter = {4}, doi = {10.1186/s12984-023-01295-5}, url = {https://doi.org/10.1186/s12984-023-01295-5}, author = {Laura Becerra-Fajardo and Jesus Minguillon and Krob, Marc O. and Camila Rogrigues and Miguel Gonz{\'a}lez-S{\'a}nchez and {\'A}lvaro Meg{\'\i}a-Garc{\'\i}a and Redondo Gal{\'a}n, Carolina and Guiti{\'e}rrez Henares, Francisco and Albert Comerma and del Ama, Antonio J. and {\'A}ngel Gil-Agudo and Francisco Grandas and Andreas Schneider and Filipe O. Barroso and Antoni Ivorra} } @article {226, title = {Dynamics of High-Density Unipolar Epicardial Electrograms During PFA}, journal = {Circulation: Arrhythmia and Electrophysiology}, year = {2023}, pages = {e011914}, chapter = {e011914}, doi = {10.1161/CIRCEP.123.011914}, url = {https://www.ahajournals.org/doi/10.1161/CIRCEP.123.011914}, author = {Gerard Amor{\'o}s-Figueras and Sergi Casabella-Ramon and Zoraida Moreno-Weidmann and Antoni Ivorra and Jose M. Guerra and Tomas Garcia-Sanchez} } @conference {232, title = {Networks of Injectable Microdevices Powered and Digitally Linked by Volume Conduction for Neuroprosthetics: a Proof-of-Concept}, booktitle = {2023 11th International IEEE/EMBS Conference on Neural Engineering (NER)}, year = {2023}, doi = {10.1109/NER52421.2023.10123743}, url = {https://ieeexplore.ieee.org/abstract/document/10123743}, author = {Laura Becerra-Fajardo and Jesus Minguillon and Albert Comerma and Antoni Ivorra} } @article {215, title = {Powering Electronic Implants by High Frequency Volume Conduction: In Human Validation}, journal = {IEEE Transactions on Biomedical Engineering}, volume = {70}, year = {2023}, month = {08/2022}, pages = {659-670}, chapter = {659}, doi = {10.1109/TBME.2022.3200409}, url = {https://ieeexplore.ieee.org/document/9864046}, author = {Jesus Minguillon and Marc Tudela-Pi and Laura Becerra-Fajardo and Enric Perera-Bel and del Ama, Antonio J. and {\'A}ngel Gil-Agudo and {\'A}lvaro Meg{\'\i}a-Garc{\'\i}a and Aracelys Garc{\'\i}a-Moreno and Antoni Ivorra} } @article {213, title = {Auricular transcutaneous vagus nerve stimulation acutely modulates brain connectivity in mice}, journal = {Frontiers in Cellular Neuroscience}, volume = {16}, year = {2022}, pages = {856855}, chapter = {856855}, doi = {10.3389/fncel.2022.856855}, url = {https://www.frontiersin.org/articles/10.3389/fncel.2022.856855/abstract}, author = {Cecilia Brambilla-Pisoni and Emma Mu{\~n}oz-Moreno and Ianire Gallego-Amaro and Rafael Maldonado and Antoni Ivorra and Guadalupe Soria and Andr{\'e}s Ozaita} } @article {214, title = {Floating EMG Sensors and Stimulators Wirelessly Powered and Operated by Volume Conduction for Networked Neuroprosthetics}, journal = {Journal of NeuroEngineering and Rehabilitation}, volume = {19}, year = {2022}, pages = {57}, chapter = {57}, doi = {10.1186/s12984-022-01033-3}, url = {https://doi.org/10.1186/s12984-022-01033-3}, author = {Laura Becerra-Fajardo and Krob, Marc O. and Jesus Minguillon and Camila Rogrigues and Christine Welsch and Marc Tudela-Pi and Albert Comerma and Filipe O. Barroso and Andreas Schneider and Antoni Ivorra} } @article {223, title = {Implantable Sensor for Measuring and Monitoring Intravascular Pressure, System Comprising Said Sensor and Method for Operating Thereof}, year = {2022}, edition = {A61B5/0215; A61B5/07; A61F2/90}, chapter = {WO 2022/184801 A1}, author = {Antoni Ivorra and Laura Becerra-Fajardo and Albert Comerma and Jesus Minguillon} } @article {209, title = {Modeling methods for treatment planning in overlapping electroporation treatments}, journal = {IEEE Transactions on Biomedical Engineering}, volume = {69}, year = {2022}, month = {09/2021}, pages = {1318 - 1327}, chapter = {1318}, doi = {10.1109/TBME.2021.3115029}, url = {https://ieeexplore.ieee.org/document/9547807}, author = {Enric Perera-Bel and Borja Mercadal and Tomas Garcia-Sanchez and Miguel A. Gonz{\'a}lez-Ballester and Antoni Ivorra} } @article {218, title = {Parametric study of Pulsed Field Ablation with biphasic waveforms in an in vivo heart model: the role of frequency}, journal = {Circulation: Arrhythmia and Electrophysiology}, volume = {15}, year = {2022}, pages = {693-705}, chapter = {693}, doi = {10.1161/CIRCEP.122.010992}, url = {https://www.ahajournals.org/doi/abs/10.1161/CIRCEP.122.010992}, author = {Tomas Garcia-Sanchez and Gerard Amor{\'o}s-Figueras and Esther Jorge and Mar{\'\i}a C. Campos and Elad Maor and Jose M. Guerra and Antoni Ivorra} } @article {216, title = {Wireless networks of injectable microelectronic stimulators based on rectification of volume conducted high frequency currents}, journal = {Journal of Neural Engineering}, volume = {19}, year = {2022}, pages = {056015}, chapter = {056015}, doi = {10.1088/1741-2552/ac8dc4}, url = {https://iopscience.iop.org/article/10.1088/1741-2552/ac8dc4}, author = {Aracelys Garc{\'\i}a-Moreno and Albert Comerma and Marc Tudela-Pi and Jesus Minguillon and Laura Becerra-Fajardo and Antoni Ivorra} } @article {206, title = {Comparing High-Frequency With Monophasic Electroporation Protocols in an In Vivo Beating Heart Model}, journal = {JACC: Clinical Electrophysiology}, volume = {7}, year = {2021}, pages = {959-964}, chapter = {959}, doi = {10.1016/j.jacep.2021.05.003}, author = {Eyal Heller and Tomas Garcia-Sanchez and Yonatan Moshkovits and Raul Rabinovici and Dvora Grynberg and Amit Segev and Samuel Asirvatham and Antoni Ivorra and Elad Maor} } @article {207, title = {Volume Conduction for Powering Deeply Implanted Networks of Wireless Injectable Medical Devices: a Numerical Parametric Analysis}, journal = {IEEE Access }, volume = {9}, year = {2021}, pages = {100594-100605}, chapter = {100594-100605}, doi = {10.1109/ACCESS.2021.3096729}, url = {https://ieeexplore.ieee.org/document/9481290}, author = {Marc Tudela-Pi and Jesus Minguillon and Laura Becerra-Fajardo and Antoni Ivorra} } @article {184, title = {Auricular transcutaneous vagus nerve stimulation improves memory persistence in na{\"\i}ve mice and in an intellectual disability mouse model}, journal = {Brain Stimulation}, volume = {13}, year = {2020}, pages = {494-498}, chapter = {494}, doi = {10.1016/j.brs.2019.12.024}, url = {https://doi.org/10.1016/j.brs.2019.12.024}, author = {Anna V{\'a}zquez-Oliver and Cecilia Brambilla-Pisoni and Mikel Domingo-Gainza and Rafael Maldonado and Antoni Ivorra and Andr{\'e}s Ozaita} } @article {187, title = {Dynamics of Cell Death After Conventional IRE and H-FIRE Treatments}, journal = {Annals of Biomedical Engineering}, volume = {48}, year = {2020}, pages = {1451{\textendash}1462}, chapter = {pages1451}, doi = {10.1007/s10439-020-02462-8}, url = {https://doi.org/10.1007/s10439-020-02462-8}, author = {Borja Mercadal and Natalie Beitel-White and Kenneth N. Aycock and Q. Castellv{\'\i} and Rafael Davalos and Antoni Ivorra} } @article {199, title = {Electrophoresis-assisted accumulation of conductive nanoparticles for the enhancement of cell electropermeabilization}, journal = {Bioelectrochemistry}, volume = {(In Press, Journal Pre-proof)}, year = {2020}, pages = {107642}, chapter = {107642}, doi = {10.1016/j.bioelechem.2020.107642}, url = {https://doi.org/10.1016/j.bioelechem.2020.107642}, author = {Amina Ghorbel and Franck M. Andr{\'e} and Lluis M. Mir and Tomas Garcia-Sanchez} } @article {198, title = {EView: An electric field visualization web platform for electroporation-based therapies}, journal = {Computer Methods and Programs in Biomedicine}, volume = {197}, year = {2020}, pages = {105682}, chapter = {105682}, doi = {10.1016/j.cmpb.2020.105682}, author = {Enric Perera-Bel and Carlos Yag{\"u}e and Borja Mercadal and Mario Ceresa and Natalie Beitel-White and Rafael Davalos and Miguel A. Gonz{\'a}lez-Ballester and Antoni Ivorra} } @article {192, title = {GaN-Based Versatile Waveform Generator for Biomedical Applications of Electroporation}, journal = {IEEE Access}, volume = {(Early Access)}, year = {2020}, doi = {10.1109/ACCESS.2020.2996426}, author = {H{\'e}ctor Sarnago and Jos{\'e} M. Burd{\'\i}o and Tomas Garcia-Sanchez and Lluis M. Mir and Ignacio {\'A}lvarez-Gariburo and {\'O}scar Luc{\'\i}a} } @article {185, title = {High-voltage pulsed electric field laboratory device with asymmetric voltage multiplier for marine macroalgae electroporation}, journal = {Innovative Food Science and Emerging Technologies}, volume = {(In press, Journal Pre-proof)}, year = {2020}, doi = {10.1016/j.ifset.2020.102288}, author = {Klimentiy Levkov and Yoav Linzon and Borja Mercadal and Antoni Ivorra and C{\'e}sar Gonz{\'a}lez and Alexander Golberg} } @conference {210, title = {In Vitro Evaluation of a Protocol and an Architecture for Bidirectional Communications in Networks of Wireless Implants Powered by Volume Conduction}, booktitle = {5th International Conference on Neurorehabilitation (ICNR2020)}, volume = {28}, year = {2020}, pages = {103-108}, publisher = {Springer Nature}, organization = {Springer Nature}, edition = {Converging Clinical and Engineering Research on Neurorehabilitation IV, Biosystems \& Biorobotics}, doi = {10.1007/978-3-030-70316-5_17}, author = {Laura Becerra-Fajardo and Jesus Minguillon and Camila Rogrigues and Filipe O. Barroso and Pons, Jos{\'e} Luis and Antoni Ivorra} } @article {188, title = {In vitro study on the mechanisms of action of electrolytic electroporation (E2)}, journal = {Bioelectrochemistry}, volume = {133}, year = {2020}, pages = {107482}, chapter = {107482}, doi = {10.1016/j.bioelechem.2020.107482}, url = {https://doi.org/10.1016/j.bioelechem.2020.107482}, author = {Nina Klein and Borja Mercadal and Michael Stehling and Antoni Ivorra} } @article {193, title = {Injectable Sensors Based on Passive Rectification of Volume-Conducted Currents}, journal = {IEEE Transactions on Biomedical Circuits and Systems}, volume = {14}, year = {2020}, pages = {867-878}, chapter = {867}, doi = {10.1109/TBCAS.2020.3002326}, url = {https://ieeexplore.ieee.org/document/9117042}, author = {Shahid Malik and Q. Castellv{\'\i} and Laura Becerra-Fajardo and Marc Tudela-Pi and Aracelys Garc{\'\i}a-Moreno and Maryam Shojaei Baghini and Antoni Ivorra} } @article {196, title = {Interleaved intramuscular stimulation with minimally overlapping electrodes evokes smooth and fatigue resistant forces}, journal = {Journal of Neural Engineering}, volume = {17}, year = {2020}, pages = {046037}, chapter = {046037}, doi = {10.1088/1741-2552/aba99e}, url = {https://doi.org/10.1088/1741-2552/aba99e}, author = {Ahmed Eladly and Jaume Del Valle and Jesus Minguillon and Borja Mercadal and Laura Becerra-Fajardo and Xavier Navarro and Antoni Ivorra} } @article {194, title = {Monitoring the molecular composition of live cells exposed to electric pulses via label-free optical methods}, journal = {Scientific Reports}, volume = {10}, year = {2020}, pages = {10471}, chapter = {10471}, doi = {10.1038/s41598-020-67402-x}, url = {https://doi.org/10.1038/s41598-020-67402-x}, author = {Antoine Azan and Marianne Grognot and Tomas Garcia-Sanchez and Lucie Descamps and Val{\'e}rie Untereiner and Guilhem Gallot and Lluis M. Mir} } @article {197, title = {Physiological changes may dominate the electrical properties of liver during reversible electroporation: measurements and modelling}, journal = {Bioelectrochemistry}, volume = {(In Press, Journal Pre-proof)}, year = {2020}, doi = {10.1016/j.bioelechem.2020.107627}, url = {https://doi.org/10.1016/j.bioelechem.2020.107627}, author = {Tomas Garcia-Sanchez and Damien Voyer and Clair Poignard and Lluis M. Mir} } @article {191, title = {Possible molecular and cellular mechanisms at the basis of atmospheric electromagnetic field bioeffects}, journal = {International Journal of Biometeorology}, volume = {(In Press, available online)}, year = {2020}, doi = {10.1007/s00484-020-01885-1}, url = {https://doi.org/10.1007/s00484-020-01885-1}, author = {Michal Cifra and Francesca Apollonio and Micaela Liberti and Tomas Garcia-Sanchez and Lluis M. Mir} } @article {189, title = {Power Transfer by Volume Conduction: In Vitro Validated Analytical Models Predict DC Powers above 1 mW in Injectable Implants}, journal = {IEEE Access}, volume = {8}, year = {2020}, pages = {37808-37820}, chapter = {37808}, doi = {10.1109/ACCESS.2020.2975597}, author = {Marc Tudela-Pi and Laura Becerra-Fajardo and Aracelys Garc{\'\i}a-Moreno and Jesus Minguillon and Antoni Ivorra} } @article {195, title = {Pulsed radiofrequency for chronic pain: in vitro evidence of an electroporation mediated calcium uptake}, journal = {Bioelectrochemistry}, volume = {136}, year = {2020}, pages = {107624}, chapter = {107624}, doi = {10.1016/j.bioelechem.2020.107624}, author = {Borja Mercadal and Rub{\'e}n Vicente and Antoni Ivorra} } @article {186, title = {Industrial Electronics for Biomedicine: A New Cancer Treatment Using Electroporation}, journal = {IEEE Industrial Electronics Magazine}, volume = {13}, year = {2019}, pages = {6-18}, chapter = {6}, doi = {10.1109/MIE.2019.2942377}, author = {{\'O}scar Luc{\'\i}a and H{\'e}ctor Sarnago and Tomas Garcia-Sanchez and Lluis M. Mir and Jos{\'e} M. Burd{\'\i}o} } @article {179, title = {Successful tumor Electrochemotherapy using Sine Waves}, journal = {IEEE Transactions on Biomedical Engineering}, volume = {67}, year = {2019}, pages = {1040-1049}, chapter = {1040}, doi = {10.1109/TBME.2019.2928645}, author = {Tomas Garcia-Sanchez and Borja Mercadal and Melanie Polrot and Adeline Muscat and H{\'e}ctor Sarnago and {\'O}scar Luc{\'\i}a and Lluis M. Mir} } @article {169, title = {Avoiding neuromuscular stimulation in liver irreversible electroporation using radiofrequency electric fields}, journal = {Physics in Medicine and Biology}, volume = {63}, year = {2018}, pages = {035027}, chapter = {035027}, doi = {10.1088/1361-6560/aaa16f}, author = {Q. Castellv{\'\i} and Borja Mercadal and Xavier Moll and Fontdevila, Dolors and Andaluz, Anna and Antoni Ivorra} } @article {159, title = {Effect of applied voltage, duration and repetition frequency of RF pulses for pain relief on temperature spikes and electrical field: A computer modeling study}, journal = {International Journal of Hyperthermia }, volume = {34}, year = {2018}, pages = {112-121}, chapter = {112}, doi = {10.1080/02656736.2017.1323122}, url = {http://dx.doi.org/10.1080/02656736.2017.1323122}, author = {El{\.z}bieta Ewertowska and Borja Mercadal and V{\'\i}ctor Mu{\~n}oz and Antoni Ivorra and Trujillo, Macarena and Berjano, Enrique} } @article {171, title = {Irreversible electroporation for the treatment of cardiac arrhythmias}, journal = {Expert Review of Cardiovascular Therapy}, volume = {16}, year = {2018}, pages = {349-360 }, chapter = {349}, doi = {10.1080/14779072.2018.1459185}, url = {https://www.tandfonline.com/doi/abs/10.1080/14779072.2018.1459185}, author = {Alan Sugrue and Elad Maor and Antoni Ivorra and Vaibhav Vaidya and Chance Witt and Suraj Kapa and Samuel Asirvatham} } @article {161, title = {Modeling Liver Electrical Conductivity during Hypertonic Injection}, journal = {International Journal for Numerical Methods in Biomedical Engineering}, volume = {34}, year = {2018}, pages = {e2904}, chapter = {e2904}, doi = {10.1002/cnm.2904}, author = {Q. Castellv{\'\i} and Patricia S{\'a}nchez-Vel{\'a}zquez and Xavier Moll and Berjano, Enrique and Andaluz, Anna and Burd{\'\i}o, Fernando and Bijnens, Bart and Antoni Ivorra} } @article {167, title = {Relation between Denaturation Time Measured by Optical Coherence Reflectometry and Thermal Lesion Depth during Radiofrequency Cardiac Ablation: Feasibility Numerical Study}, journal = {Lasers in surgery and medicine}, volume = {50}, year = {2018}, pages = {222-229}, chapter = {222}, doi = {10.1002/lsm.22771}, author = {Gonz{\'a}lez-Su{\'a}rez, Ana and Herranz, David and Berjano, Enrique and Rubio-Guivernau, Jose L and Margallo-Balb{\'a}s, Eduardo} } @article {165, title = {Avoiding nerve stimulation in irreversible electroporation: a numerical modeling study}, journal = {Physics in Medicine and Biology}, volume = {62}, year = {2017}, pages = {8060-8079}, chapter = {8060}, doi = {https://doi.org/10.1088/1361-6560/aa8c53}, author = {Borja Mercadal and Christopher Arena and Rafael Davalos and Antoni Ivorra} } @article {166, title = {Focused Transhepatic Electroporation Mediated by Hypersaline Infusion throuth the Portal Vein in Rat Model. Preliminary Results on Differential Conductivity}, journal = {Radiology and Oncology}, volume = {51}, year = {2017}, pages = {415-421}, chapter = {415}, author = {Clara Pa{\~n}ella and Q. Castellv{\'\i} and Xavier Moll and Rita Quesada and Alberto Villanueva and M. Iglesias and Dolores Naranjo and Patricia S{\'a}nchez-Vel{\'a}zquez and Andaluz, Anna and Luis Grande and Antoni Ivorra and Burd{\'\i}o, Fernando} } @article {158, title = {Long-term effectiveness of irreversible electroporation in a murine model of colorectal liver metastasis}, journal = {Scientific reports}, volume = {7}, year = {2017}, chapter = {44821}, doi = {10.1038/srep44821}, author = {Patricia S{\'a}nchez-Vel{\'a}zquez and Q. Castellv{\'\i} and Alberto Villanueva and M. Iglesias and Rita Quesada and Clara Pa{\~n}ella and Marta C{\'a}ceres and Dimitri Dorcaratto and Andaluz, Anna and Xavier Moll and Jos{\'e} M. Burd{\'\i}o and Luis Grande and Antoni Ivorra and Burd{\'\i}o, Fernando} } @inbook {155, title = {Assessment of Electroporation by Electrical Impedance Methods}, booktitle = {Handbook of Electroporation}, year = {2016}, pages = {1-20 (electronic)}, publisher = {Springer International Publishing}, organization = {Springer International Publishing}, abstract = {

Electroporation causes an immediate increase in cell membrane permeability that results in membrane conductivity increase, which has an effect in the measured impedance of the cell suspension or the tissue. Therefore, impedance measurements offer the possibility to perform real-time assessment of the electroporation phenomenon in a minimally invasive fashion. Nevertheless, impedance measurements in biological organisms depend on many factors and other processes besides the membrane permeabilization. This lack of specificity can be an important drawback for using impedance measurements as an electroporation measure. An equivalent electrical model of cell suspensions and tissues is commonly employed to better understand how the different processes that take place during electroporation can affect the measured impedance of a sample. This chapter briefly overviews the information that can be extracted from impedance measurements during and after the application of electroporation pulses. These measurements have been widely used to observe and analyze the dynamics of the phenomenon. Impedance has the potential to be used as a tool to assess electroporation effectiveness of treatment. A significant conclusion from the experimental studies on the topic is that conductivity measured shortly after treatment appears to be correlated with electroporation effectiveness in terms of cell membrane permeabilization. That is, it has the potential to be used as an electroporation effectiveness indicator. On the other hand, dynamic conductivity during the electroporation pulses, which is much easier to be measured, does not seem to be correlated with electroporation effectiveness.

}, issn = {978-3-319-26779-1}, doi = {10.1007/978-3-319-26779-1_164-1}, author = {Q. Castellv{\'\i} and Borja Mercadal and Antoni Ivorra} } @article {151, title = {Dependence of electroporation detection threshold on cell radius: an explanation to observations non compatible with Schwan{\textquoteright}s equation model}, journal = {Journal of Membrane Biology}, volume = {249}, year = {2016}, pages = {663-676}, chapter = {663}, abstract = {

It is widely accepted that electroporation occurs when the cell transmembrane voltage induced by an external applied electric field reaches a threshold. Under this assumption, in order to trigger electroporation in a spherical cell, Schwan{\textquoteright}s equation leads to an inversely proportional relationship between the cell radius and the minimum magnitude of the applied electric field. And, indeed, several publications report experimental evidences of an inverse relationship between the cell size and the field required to achieve electroporation. However, this dependence is not always observed or is not as steep as predicted by Schwan{\textquoteright}s equation. The present numerical study attempts to explain these observations that do not fit Schwan{\textquoteright}s equation on the basis of the interplay between cell membrane conductivity, permeability and transmembrane voltage. For that, a single cell in suspension was modeled and it was determined the electric field necessary to achieve electroporation with a single pulse according to two effectiveness criteria: a specific permeabilization level, understood as the relative area occupied by the pores during the pulse, and a final intracellular concentration of a molecule due to uptake by diffusion after the pulse, during membrane resealing. The results indicate that plausible model parameters can lead to divergent dependencies of the electric field threshold on the cell radius. These divergent dependencies were obtained through both criteria and using two different permeabilization models. This suggests that the interplay between cell membrane conductivity, permeability and transmembrane voltage might be the cause of results which are non compatible with the Schwan{\textquoteright}s equation model.\ 

}, author = {Borja Mercadal and P. Thomas Vernier and Antoni Ivorra} } @article {150, title = {Irreversible electroporation of the liver: is there a safe limit to the ablation volume?}, journal = {Scientific Reports}, volume = {6}, year = {2016}, pages = {23781}, doi = {10.1038/srep23781}, author = {Patricia S{\'a}nchez-Vel{\'a}zquez and Q. Castellv{\'\i} and Alberto Villanueva and Rita Quesada and Clara Pa{\~n}ella and Marta C{\'a}ceres and Dimitri Dorcaratto and Andaluz, Anna and Xavier Moll and Trujillo, Macarena and Jos{\'e} M. Burd{\'\i}o and Berjano, Enrique and Luis Grande and Antoni Ivorra and Burd{\'\i}o, Fernando} } @article {141, title = {Comparison of the effects of the repetition rate between microsecond and nanosecond pulses: Electropermeabilization-induced electro-desensitization?}, journal = {Biochimica et Biophysica Acta (BBA) - General Subjects}, volume = {1840}, year = {2014}, pages = {2139 - 2151}, keywords = {Electroporation}, issn = {0304-4165}, doi = {http://dx.doi.org/10.1016/j.bbagen.2014.02.011}, url = {http://www.sciencedirect.com/science/article/pii/S0304416514000725}, author = {Aude Silve and A. Guimer{\`a} Brunet and B. Al-Sakere and Antoni Ivorra and L.M. Mir} } @article {142, title = {In vivo assessment of corneal barrier function through non-invasive impedance measurements using a flexible probe}, journal = {Journal of Physics: Conference Series}, volume = {434}, year = {2013}, pages = {012072}, abstract = {

The cornea is a transparent structure composed of three layers: the epithelium, the stroma and the endothelium. To maintain its ransparency the stroma remains in a constant state of dehydration. Consequently, any ion flow disorder through the covering layers can compromise the barrier function and, therefore the corneal homeostasis. Since ionic permeability has a fundamental impact on the passive electrical properties of living tissues, in this work it is proposed and demonstrated a diagnosis method based on tetrapolar impedance measurements performed by electrodes placed on the corneal surface. The contribution of each cornea layer to the total measured impedance has been analysed over a frequency range. Following the obtained guidelines, a flexible probe with integrated electrodes has been developed and manufactured using SU-8 photoresin. The feasibility of the proposed method has been evaluated in vivo by monitoring corneal epithelium wound healing. Obtained impedance measurements have been compared with measurements of permeability to sodium fluorescein from different excised corneas. Successful results demonstrate the feasibility of this novel flexible sensor and its capability to quantify corneal permeability in vivo in a noninvasive way.

}, url = {http://stacks.iop.org/1742-6596/434/i=1/a=012072}, author = {A Guimera and X Illa and E Traver and S Marchan and C Herrero and C Lagunas and M J Maldonado and Antoni Ivorra and R Villa} } @conference {Silve2011, title = {{Detection of permeabilisation obtained by micropulses and nanopulses by means of bioimpedance of biological tissues}}, booktitle = {5th European Conference on Antennas and Propagation (EUCAP)}, year = {2011}, pages = {3164{\textendash}3167. {\textcopyright} 2011 Institute of Electrical and Electronics Engineers, Inc.}, address = {Rome, Italy}, abstract = {

In this paper permeabilisation of potato tissue caused by either microsecond electric pulses or nanosecond electric pulses is compared. The intensity of permeabilisation is quantified by means of bio-impedance change. Thanks to this method, the impact of the repetition frequency was investigated. Data show that very low repetition frequencies can be much more efficient to permeabilise.

}, author = {Aude Silve and Antoni Ivorra and L.M. Mir} } @article {Ivorra2010a, title = {{Electric field redistribution during tissue electroporation: its potential impact on treatment planning}}, journal = {Comptes Rendus Physique}, volume = {Accepted (still pending publication)}, year = {2010}, abstract = {
Electroporation is the phenomenon in which cell membrane permeability is increased by exposing the cell to short high electric field pulses. Electroporation is accompanied by an increase of tissue electrical conductivity during the pulses. Such conductivity increase results in a redistribution of the electric field magnitude that can be simulated with simple functions describing the change in tissue conductivity. Experiments on potato tuber reveal that the conductivity increase phenomenon has indeed a significant impact on field distribution, and validate the use of models that take into account such conductivity alteration. For instance, the error in electroporated area estimation can decrease from 30 \% to 3 \%.\ 
}, author = {Antoni Ivorra and Boris Rubinsky and L.M. Mir} } @article {Ivorra2010, title = {{Electrical modeling of the influence of medium conductivity on electroporation.}}, journal = {Physical chemistry chemical physics : PCCP}, volume = {12}, number = {34}, year = {2010}, pages = {10055{\textendash}64}, abstract = {

Electroporation is the phenomenon in which cell membrane permeability is increased by exposing the cell to short high electric field pulses. Experimental data show that the amount of permeabilization depends on the conductivity of the extracellular medium. If medium conductivity decreases then it is necessary to deliver a pulse of larger field amplitude in order to achieve the same effect. Models that do not take into account the permeabilization effect on the membrane conductivity cannot reproduce qualitatively the experimental observations. Here we employ an exponential function for describing the strong dependence of membrane conductivity on transmembrane potential. Combining that model with numerical methods we demonstrate that the dependence on medium conductivity can be explained as being the result of increased membrane conductance due to electroporation. As experimentally observed, extracellular conductivities of about 1 and 0.1 S m(-1) yield very similar results, however, for lower conductivities (\<0.01 S m(-1)) the model predicts that significantly higher field magnitudes will be required to achieve the same amount of permeabilization.

}, keywords = {Biological, Cell Membrane, Cell Membrane Permeability, Cell Membrane: metabolism, Diffusion, Electric Conductivity, Electroporation, Membrane Potentials, Models, Reproducibility of Results}, issn = {1463-9084}, doi = {10.1039/c004419a}, url = {http://www.ncbi.nlm.nih.gov/pubmed/20585676}, author = {Antoni Ivorra and J. Villemejane and L.M. Mir} } @inbook {Silve2010, title = {{Nanosecond pulsed electric field delivery to biological samples: difficulties and potential solutions}}, booktitle = {Advanced Electroporation Techniques in Biology and Medicine}, year = {2010}, pages = {353{\textendash}370}, abstract = {

In this chapter we discuss particular features of the nanosecond electric pulsed fields (nsPEFs) that must be taken into account when experimenting on their delivery to biological samples. The purpose of the chapter is to provide future users of this technology with some advice on how to correctly apply it. It is first analyzed how propagation related phenomena can impact on the actual electric field pulse that is applied to the sample. In particular, it is shown that impedance matching for the exposure chamber will be a key element. It is then proposed to employ monitoring systems for voltage and current signals and some indications about their use and limitations are given. Finally, the electrochemical and thermal consequences of nsPEFs delivery are also discussed. We conclude that only excellent experimental conditions can result in robust, controlled and reproducible data on the nsPEFs biological effects.

}, keywords = {breakdown of dielectric, electrochemical reactions, impedance, matching, materials, monitoring, nanosecond electric pulses, thermal effects, transmission line, waves propagation}, isbn = {978-1-439-81906-7}, author = {Aude Silve and J. Villemejane and Joubert, V. and Antoni Ivorra and L.M. Mir} }